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The efficiency of Paenibacillus mucilaginosus bacteria cultivation on nutrient medium based on sucrose

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Silicate bacteria or Paenibacillus mucilaginosus are one of the most perspective microorganisms in soil for obtaining biological products as microbiological fertilizers stimulating plant growth. Biological products of P. mucilaginosus are recommended for use in various fields, as a biosorbent for wastewater treatment, and also in the form of feed additives as stimulating in animal husbandry. The aim of the study is to determine the efficiency of cultivation of P. mucilaginosus bacteria strains on nutrient medium from sucrose. Six strains of P. mucilaginosus bacteria were used in this study: 560, 563, 567, 568, 572, an 574, provided for research by All-Russia Research Institute for Agricultural Microbiology (St. Petersburg). They were cultivated on the Alexandrov’s mineral nutrient medium using sucrose as a carbon source. It was established that the cultivation of mucilaginosus bacteria strains on nutrient medium from sucrose demonstrates a double growth cycle (except for strain 568). Moreover, these bacteria have β-fructofuranosidase and catalase activity, fix nitrogen from the air, and produce indole-3-acetic acid. The most productive strain of P. mucilaginosus bacteria is strain 574. The effect of sodium chloride concentration and temperature on the growth of P. mucilaginosus bacteria is studied. The strain 568 of P. mucilaginosus bacteria is shown to have moderately halophilic properties with mesophilic cultivation temperature parameters. In addition, during the cultivation on a nutrient medium from sucrose P. mucilaginosus bacteria synthesize exopolysaccharides, the maximum yield of which is reachedafter 48 hours. Further cultivation of bacteria results in the destruction of exopolysaccharides by glycolytic enzymes, which are synthesized by P. mucilaginosus bacteria.

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