Enzymatic hydrolysis of integumentary tissues of pike perch
Annotation
Russia is among the top ten countries in the extraction of aquatic biological resources. The potential of secondary fish raw materials accumulated during the cutting of fish is not fully disclosed at present. On the territory of the Kaliningrad region the most promising sources of research are: pike perch, cod, bream, herring, and sprat. The purpose of this work is to evaluate the effectiveness of the use of enzymes for processing products of pike-perch cutting, namely the integumentary tissue. The integumentary tissue makes up 2-7% of the total mass of fish and consists of a significant amount of collagen proteins. In this study, the parameters of enzymatic hydrolysis of pike-perch integumentary tissues were substantiated using the following proteolytic enzyme preparations: Papain (manufactured by Animox, Germany) and the enzyme Alcalase® 2,4 L from Novozymes, Denmark. Alkalase). According to the manufacturer, both enzymes are most active at a temperature of 50 ° and neutral pH 6-7, which corresponds to the natural pH of fish raw materials. The amount of the introduced enzyme preparation was varied to achieve the highest yield of the final product. The influence of the duration of the process was analyzed. To justify the time of fermentolysis by formative titration, the amount of accumulated non-protein amine nitrogen in the protein extract was estimated. The degree of hydrolysis is a criterion for the effectiveness of enzyme preparations. The growth of protein breakdown products in the soluble fraction and non-hydrolyzed substrate was evaluated. During the time of fermentolysis, in increments of 30 minutes, the amount of dry substances was determined. Fermentolysis was carried out for 12 hours with constant stirring and a 1:3 hydromodule. According to the results of the studies carried out for the enzymatic hydrolysis of the integumentary tissues of fish, the choice of the enzyme preparation Papain is justified: at the dosage of the enzyme of 1.5% by weight of the raw material and carrying out fermentolysis for 3.5 hours, the highest indicator of the degree of hydrolysis of the raw material (to 75%) was obtained.
Keywords
Постоянный URL
Articles in current issue
- Refrigeration supply systems based on lithium bromide absorption refrigerating machines
- Heat transfer intensity of heat exchangers with plate fins
- The efficiency of the methods for regulating the capacity of scroll compressor as a part of the refrigeration system
- The scheme of liquefied carbon dioxide production by membrane separation with recirculation system
- Energy modeling and experimental validation of heat pump operating modes in exhaust air heat recovery. Part 2. Energy, economic and environmental indicators
- The process of fat extraction from fish processing waste as a raw material for biotechnological synthesis of polyhydroxyalkanoates
- Quick-freezing of raw-materials of animal origin using cryogenic technologies
- Influence of ultrasound on freezing and the quality of frozen fruits and vegetables
- Fundamental equation of state for propylcyclohexane
- Swirling vapour flow in short linear heat pipes. Part I