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Storage stability and DPPH scavenging capacity of polyphenols of Mongolia Scotch Pine
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Mongolia scotch pine have recently received great attention for their health benefits, mainly due to their high polyphenols content. Nevertheless, stability of pine polyphenols in liquid solution and DPPH scavenging ability during the process of storage have been only little investigated. In this present study, Mongolia scotch pine cone peer was used as raw material, effect of different storage conditions on polyphenol stability were studied, based on color and DPPH free radical scavenging capacity of polyphenols change. Polyphenols contents were quantified with Folin-Ciocalteu. L*, a* and b* values were detected using Color Companion software and antioxidant ability was evaluated by DPPH scavenging capacity. The results showed that polyphenols decreased 16%, 91.3% and 97.2% at 4 deg С, 25 deg С and 40 deg С respectively during 5weeks storage time and degradation followed first-order kinetics. The Activation energy was 68.98kJ/mol-1. Polyphenols were stable at neutral and alkaline environment. The color analysis revealed that color parameters (L*, a*, b* and TCD) were assumed to have an Arrhenius-type dependence on temperature, model followed a first-order reaction. The DPPH free radical scavenging capacity of phenolics is affected by storage temperature, pH and time, and it reached the highest value at 1th week. This research highlights the importance of considering conditions when preserving polyphenols. In cool, neutral and alkaline environment, the polyphenols were stable for 5 weeks.
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