Dynamics of biosynthesis of beta-glucans by Aspergillus niger, Streptomyces lucensis, and Streptomyces violaceus microorganisms during their cultivation on various media
Annotation
We studied the formation of beta-glucans in Aspergillus niger VKPM F-171 micromycetes, Streptomyces lucensis VKPM Ac-1743 micromycetes, and Streptomyces violaceus VKPM Ac-1734. The objects of study were the biomass of Asregillus niger VKPM F-171, Streptomyces lucensis VKPM As-1743, and Streptomyces violaceus VKPM As-1734 micromycetes, obtained by fermentation of starch-containing (starch hydrolysates and sweet sugar, grain and cereal grits) hydrolysates of microbial biomass. The content of β-glucans in the biomass of micromycetes was determined in accordance with GOST 57513-2017. In the hydrolysates obtained, the content of glucose, disaccharides (in terms of maltose), and polysaccharides (in terms of dextrins) was determined by the Sicherd-Blayer method modified by Smirnov. The amino nitrogen content was determined by the iodometric method. It was shown that the total glucan content in the biomass obtained by fermentation of a corn starch hydrolyzate with Aspergillus niger VKPM F-171 strain is 1.5-2 times higher in comparison with other biomass samples under investigation. An increased amount of β-glucans was also detected in the biomass of Aspergillus niger VKPM F-171 micromycete during the fermentation of corn starch hydrolyzate. β-Glucans in the biomass of strains of Streptomyces lucensis VKPM Ac-1743 and Streptomyces violaceus VKPM Ac-1734 are mainly formed upon cultivation on a sugar-mineral medium. It was shown that the content of β-glucans in Aspergillus niger VKPM F-171, Streptomyces lucensis VKPM Ac-1743, and Streptomyces violaceus VKPM Ac-1734 cells depends on the chemical nature of the substrate. The results expand the understanding of the formation of glucan-containing compounds in the biomass of micromycetes of Aspergillus and Steptomyces genus; they are the initial stage in the development of a biotechnological method for producing β-(1,3)- and β-(1,3)(1,6)-glucans from microbial biomass. They create the prospect of obtaining a microbial-plant substance with a high content of glucan-containing compounds with a predominance of (1→3)-β-D- and β-(1→3)(1→6)-β-D-glucans.
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